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Posted on: Aug 28, '08

Anti Nuclear Antibodies (ANA)

Anti Nuclear Antibodies (ANA)

The presence of one of more circulating serum autoantibodies to nuclear antigens is a hallmark of Systemic Rheumatic
Diseases. A negative ANA test virtually rules out a diagnosis of Systemic Lupus Erythematosus but a positive test
may be indicative of a number of autoimmune connective tissue diseases such as Scleroderma, Rheumatoid Arthritis and
Sjogren’s syndrome. ANA test carried out by Immunofluorescence assay using HEP-2 slide (Tissue culture substrate) is
more sensitive and specific than ANA carried out by enzyme immunoassay.

The ANA pattern seen on immunofluroscence staining helps in determination of the antibody specificities, which need
to be confirmed by immunoblot techniques.

A result reported in primary dilution of 1:40 does not preclude a stronger response. A specimen that is tested at
1:40 with an intensity of 2+ or greater needs a higher dilution, to get end titre readings.

The positivity seen on fluorescence indicates

1+ Positivity = Weakly positive, can be found in normal healthy individual (1:20).

2+ Positivity = Moderate positive, clinically insignificant in most cases (1:40).

3+ Positivity = Significant positive, needs clinical correlation (1:100).

4+ Positivity = Strong positive, highly suggestive of collagen vascular disease (1:1000).

Titre estimation helps to monitor response to treatment.

Methodology of Test: Immunofluroscence.

Tags: news, ayan

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CancerSA said:

Interesting post!

August 30, '08

manocarla said:
tx very interesting

August 29, '08

magsvir2000 said:

August 29, '08

VintageWine48 said:
Very useful information. I wish I could work with you on a full time basis to develop medicines, since your scientific background would serve as an ideal foil to my layman ideas. I continue to pray for your father. How is he now? Great post.

August 28, '08

daisyann26 said:

August 28, '08

geetsudha said:

August 28, '08

tenjade_destiny said:
hmmm, thx

August 28, '08

friendship1577 said:
Thanks for sharing.

August 28, '08

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